TABLE 1.
Abbreviated list of bacterial strains and plasmids used in this studya
| Bacterium and strain | Genotype | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | supE44 ΔlacU169 (80lacZΔM15)hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Laboratory collection |
| DY330 | W3110 ΔlacU169 gal490 λcI857 Δ(cro-bioA) | 31 |
| pGEM-T Easy | Promega | |
| pKD3 (FRT-cat-FRT allele) | 10 | |
| pKD4 (FRT-kan-FRT allele) | 10 | |
| MB838 | DH5αλpir pR6KγFRT-gent-FRT | This work |
| MB790 | DH5α pMMBFlp | This work |
| MB791 | DH5α pBSFlp | This work |
| MB750 | DH5α pGEMfliA | This work |
| MB751 | DH5α pGEMΔfliA::FRT-cat-FRT | This work |
| L. pneumophila strains | ||
| MB110 | Lp02 wild type; thyA hsdR rpsL (Strr) | 1 |
| MB811 | Lp02 ΔfliA::FRT-cat-FRT | This work |
| MB818 | Lp02 ΔfliA::FRT | This work |
| MB758 | Lp02 recJ::FRT-cat-FRT | 1a |
| MB819 | Lp02 ΔrecJ::FRT | This work |
| MB759 | Lp02 xseA::FRT-kan-FRT | 1a |
| MB820 | Lp02 ΔxseA::FRT | This work |
| MB760 | Lp02 recJ::FRT-cat-FRT xseA::FRT-kan-FRT | 1a |
| MB821 | Lp02 recJ::FRT xseA::FRT | This work |
a
See Table S1 for a complete list of strains.