TABLE 2.
amoA gene sequences and OTU biofilter sample summary
| Samplea | No. of amoA gene sequences in: |
|
|---|---|---|
| Biofilter OTU 1 | Biofilter OTU 2 | |
| A1 (influent end) | 105 | 25 |
| A2 | 67 | 3 |
| A3 | 8 | 1 |
| A4 (effluent end) | 14 | 0 |
| Total for A | 194 | 29 |
| C1 (influent end) | 73 | 0 |
| C2 | 74 | 0 |
| C3 | 73 | 1 |
| C4 (effluent end) | 65 | 0 |
| Total for C | 285 | 1 |
| Total for A and C | 479 | 30 |
a
The influent end of the biofilter was designated section 1, with the numbering progressing down the length of the biofilter in equal lengths such that the effluent end was designated section 4. Each section was divided into three subsamples that were used for DNA extraction, resulting in triplicate extractions for each section. For the extractions, the mass of dry anthracite used was 0.60 ± 0.06 g (mean ± standard deviation) per extraction (wet anthracite moisture content, 32% ± 0.74% [mean ± standard deviation]).