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. 2011 Feb 11;77(7):2534–2536. doi: 10.1128/AEM.01731-10

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FIG. 1. — Colony PCR analysis of the pyrF (A), pta ack (B), and ldh (C) loci during construction of the markerless ethanologen strain. (D) Diagram showing genomic modifications, transforming vectors, and selection conditions. Note that the pyrF deletion is not essential for creation of the markerless ethanologen strain M0355. PCR was performed with primers external to areas of homologous recombination. For each panel: lane 1, wild-type YS485; lane 2, M0229 (ΔpyrF); lane 3, M0350 (ΔpyrF Δpta Δack); lane 4, M0350(pMU424) (ΔpyrF Δpta Δack Δldh::pta ack Kan^r); lane 5, M0353 (ΔpyrF Δpta Δack Δldh); lane 6, M0355 (Δpta Δack Δldh). The DNA size marker is a 1-kb New England BioLabs DNA ladder.