TABLE 1.
Genes and primers used for qRT-PCR
| ORF | Gene | Primer sequence(5′→3′) |
Reference | |
|---|---|---|---|---|
| Forward | Reverse | |||
| EF0900 | adhE | TCTGAGCAAGCGGTCCATTGTGG | AGTCGAATTAGAAGGTGCAGGTCCAG | This study |
| EF1612 | pflA | CCAGGTGTCCGTTTTATCGTATTTAC | GGCATTCATAACAACCTTAGATACG | This study |
| EF0082 | GCTTGCACGACTTTTCATGGGGAAAC | GGGCCATTTATTGGGATGTTATTG | This study | |
| EF1964 | gap-2 | TAATGACAACTATCCACGCTTACAGG | CTTTTGTTTGAGTTGCATCGAATGAACC | This study |
| EF0255 | ldh-1 | CGCAGGGAATAAAGATCACCA | GCAATCGTCATAAGTAGCAGCA | This study |
| 23S rRNA | CCTATCGGCCTCGGCTTAG | AGCGAAAGACAGGTGAGAATCC | 26 | |