TABLE 3.

Significantly upregulated genes in the mutant, as identified by microarray

ORF	Gene	Putative function	Functional category	Amt of upregulation in mutant (log2 value)
EF0255a	ldh-1	l-Lactate dehydrogenase	Energy metabolism	3.24
EF0552		PTS system, IIC component	Energy metabolism	1.21
EF0677		Phosphoglucomutase/phosphomannomutase family protein	Energy metabolism	1.67
EF0806		Amino acid ABC transporter, permease protein	Transport and binding	1.09
EF0900	adhE	Bifunctional acetaldehyde-CoA/alcohol dehydrogenase	Energy metabolism	3.57
EF0949	eutD	Phosphotransacetylase	Energy metabolism	1.22
EF1017		PTS system, IIB component	Signal transduction	1.42
EF1018		PTS system, IIA component	Signal transduction	1.30
EF1019		PTS system, IIC component	Signal transduction	1.84
EF1213	alsS	Acetolactate synthase	Energy metabolism	1.51
EF1214	budA	Alpha-acetolactate decarboxylase	Energy metabolism	1.92
EF1343		Sugar ABC transporter, permease protein	Transport and binding	1.50
EF1353	pdhA	Pyruvate dehydrogenase complex E1 component, alpha subunit	Energy metabolism	2.65
EF1354	pdhB	Pyruvate dehydrogenase complex, E1 component, beta subunit	Energy metabolism	2.45
EF1355	aceF	Dihydrolipoamide acetyltransferase	Energy metabolism	2.29
EF1356	lpdA	Dihydrolipoamide dehydrogenase	Energy metabolism	2.51
EF1612	pflA	Pyruvate formate lyase activating enzyme	Energy metabolism	1.22
EF1613	pflB	Formate acetyltransferase	Energy metabolism	1.44
EF1712	pyrE	Orotate phosphoribosyltransferase	Purine, pyrimidine, nucleoside, and nucleotide biosynthesis	4.07
EF1713	pyrF	Orotidine 5′-phosphate decarboxylase	Purine, pyrimidine, nucleoside, and nucleotide biosynthesis	4.19
EF1714	pyrD2	Dihydroorotate dehydrogenase	Purine, pyrimidine, nucleoside, and nucleotide biosynthesis	2.10
EF1718	pyrC	Dihydroorotase	Purine, pyrimidine, nucleoside, and nucleotide biosynthesis	4.73
EF1719	pyrB	Aspartate carbamoyltransferase catalytic subunit	Purine, pyrimidine, nucleoside, and nucleotide biosynthesis	3.50
EF1720		Uracil permease	Purine, pyrimidine, nucleoside, and nucleotide biosynthesis	1.90
EF2213		PTS system, IIBC components	Energy metabolism	1.64
EF3014		Cation transporter E1-E2 family ATPase	Transport and binding	1.56
EF3199		ABC transporter, permease protein	Transport and binding	3.17
EF3200		ABC transporter, ATP-binding protein	Unknown function	2.61
EF3315		Triphosphoribosyl-dephospho-CoA synthase	Unknown function	1.34
EF3318	citX	2′-(5″-Triphosphoribosyl)-3′-dephospho-CoA:apo-citrate lyase	Energy metabolism	1.59
EF3319	citF	Citrate lyase, alpha subunit	Energy metabolism	0.92
EF3320	citE	Citrate lyase, beta subunit	Energy metabolism	1.66
EF3321	citD	Citrate lyase, gamma subunit	Energy metabolism	1.74
EF3322	citC	Citrate lyase ligase	Energy metabolism	1.24
EF3324		Sodium ion-translocating decarboxylase, beta subunit	Energy metabolism	2.47
EF3325		Sodium ion-translocating decarboxylase/biotin carboxyl carrier protein subunit	Energy metabolism	2.17
EF3327		Citrate transporter	Transport and binding	2.09
EFB0038		Conserved hypothetical protein	Hypothetical protein	1.03
EFB0042		Hypothetical protein	Hypothetical protein	6.08
EFB0043	ssb-6	Single-strand-binding protein	DNA metabolism	6.31
EFB0044		Hypothetical protein	Hypothetical protein	5.84
EFB0045	nuc-2	Thermonuclease precursors	DNA metabolism	6.16
EFB0046		Conserved domain protein	Hypothetical protein	6.12

^aThe gene is truncated in the mutant.