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. 2011 Feb 4;77(7):2522–2526. doi: 10.1128/AEM.02789-10

FIG. 2.

FIG. 2.

Construction and replication of plasmids bearing cognate or heterologous IncP-1 replication elements. (A) Locations and sources of IncP-1 replication module elements (trfA and oriV). Plasmid names are indicated on the left. Regions derived from pXF-RIV11 are indicated as white boxes; regions derived from pVEIS01 are indicated as gray boxes. Locations of relevant endonuclease restriction sites are indicated; distances in base pairs separating endonuclease restriction sites are indicated parenthetically. (B and C) Restriction endonuclease profiles of plasmids bearing cognate (pXF-S-XF and pVE-S-VE) or heterologous (pXF-S-VE and pVE-S-XF) IncP-1 replication module elements and used to transform Xylella fastidiosa (O) or rescued from X. fastidiosa by transformation of Escherichia coli (R). Note that length polymorphism of fragments bearing trfA (B) or oriV (C) verifies the source (XF = pXF-RIV11; VE = pVEIS01) of the respective IncP-1 replication module elements.