Fig. 1.
Growth inhibition of procyclic and bloodstream life cycle stages of T. brucei by 2-bromopalmitate. T. brucei cultures were initiated at a density of 1 × 106/ml for strain 29-13 procyclic parasites or 1 × 105/ml for single-marker strain bloodstream-form parasites. After 24 h in the presence of various concentrations of the palmitoylation inhibitor 2-bromopalmitate or vehicle control, parasite density was determined by Neubauer hemocytometry. Cell density was normalized to the vehicle control, and data are presented as the means ± standard errors of the means of triplicate experiments.