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Depletion of individual PAT mRNAs by RNA interference. Semiquantitative RT-PCR was conducted on RNA samples isolated from either parental 29-13 (wild-type [WT]) cells, or individual PAT RNAi cell lines cultured with or without tetracycline for 2 days. Labels along the vertical axes indicate the primer set used to monitor mRNA levels, with α-tubulin serving as a control.
