Fig. 4.

Gene expression patterns in cells depleted of Swi6p and Swi4p. (A) Northern analysis demonstrating downregulation of G₁ cyclins CCN1 and PCL2 in cells depleted of Swi6p and Swi4p. A total of 20 μg of RNA from strains BH420 (SWI4/SWI4 SWI6/SWI6 URA3⁺ HIS1⁺ ARG4⁺), AG168 (swi4Δ::hisG/swi4Δ::URA3 swi6Δ::ARG4/swi6Δ::HIS1), and BH190 (swi6Δ::URA3/swi6Δ::HIS1 swi4Δ::hisG/MET3::SWI4-ARG4) grown under repressing conditions was loaded on the gel. ACT1 was used a loading control. (B) Venn diagrams comparing significantly modulated genes in strain BH190 with the total number of genes in C. albicans that show cell cycle-dependent periodic expression patterns (26). Strains BH190 and BH420 were incubated in repressing medium for 7 h and processed for microarray analysis. Data obtained from four microarray chips representing four separate samples were normalized with Lowess using GeneSpring software. Significantly modulated genes were determined based on a 1.7-fold cutoff and t test function with a P value of <0.05. (C) Pie categorization of significantly modulated genes in cells depleted of Swi4p and Swi6p. Gene names and functions were identified through GeneSpring analysis and updated using the Candida Genome Database (CGD) at http://candidagenome.org/. Different colors represent different categories as indicated. Genes were manually categorized according to a single function, although several have multiple functions.