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Expression of GFP-tagged Mep2 in mep1Δ mep2Δ double mutants (strains SCΔmep12MEP2G7A and -B) and mep1Δ mep2Δ npr1Δ triple mutants (strains SCΔmep12Δnpr1MEP2G7A and -B). Cells were grown in SD medium containing 100 μM ammonium at 30°C and observed by fluorescence microscopy. The two independently generated series of mutants behaved identically, and only one of them is shown in each case.
