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Growth of the wild-type strain SC5314, mep1Δ mep2Δ double mutants, and mep1Δ mep2Δ mep3Δ triple mutants on different concentrations of ammonium as the sole nitrogen source. Tenfold dilution series of the strains were spotted on SD plates containing the indicated ammonium concentrations and incubated for 3 days at 30°C. The following strains were used: SC5314 (wild type), SCMEP12M4A and -B (mep1Δ mep2Δ), and SCMEP123M4A and -B (mep1Δ mep2Δ mep3Δ).
