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. 2011 Mar;10(3):332–342. doi: 10.1128/EC.00293-10

Fig. 7.

Fig. 7.

(A) Growth of the wild-type strain SC5314, mep1Δ mep2Δ double mutants, mep1Δ mep2Δ npr1Δ triple mutants, and strains in which a wild-type copy of MEP2 or the MEP2G343C allele was reinserted on different concentrations of ammonium as the sole nitrogen source. Tenfold dilution series of the strains were spotted on a YPD control plate or on SD plates containing the indicated ammonium concentrations and incubated for 1 day (YPD) or 3 days (SD) at 30°C. The following strains were used: SC5314 (wild type), SCMEP12M4A and -B (mep1Δ mep2Δ), SCΔmep12MEP2K1A and -B (mep1Δ mep2Δ + MEP2), SCΔmep12MEP2K2A and -B (mep1Δ mep2Δ + MEP2G343C), Δmep12NPR1M4A and -B (mep1Δ mep2Δ npr1Δ), Δmep1Δmep2Δnpr1MEP2K1A and -B (mep1Δ mep2Δ npr1Δ + MEP2), and Δmep1Δmep2Δnpr1MEP2K2A and -B (mep1Δ mep2Δ npr1Δ + MEP2G343C). (B) Ammonium uptake by the same strains. Uptake rates were determined in the presence of 1 mM ammonium as described in Materials and Methods. Shown are the means and standard deviations from three independent experiments (two with the A series and one with the B series of mutants). Data are from the same experiments with results shown in Fig. 2, and the values of the control strains are included for comparison.