Table 1.
C. albicans strains used in this study
| Strain | Parent | Relevant characteristic or genotypea | Reference or source |
|---|---|---|---|
| SC5314 | Wild-type parental strain | 12 | |
| npr1Δ mutants and complemented strains | |||
| NPR1M1A | SC5314 | npr1-1::SAT1-FLIP/NPR1-2 | This study |
| NPR1M1B | SC5314 | NPR1-1/npr1-2::SAT1-FLIP | This study |
| NPR1M2A | NPR1M1A | npr1-1::FRT/NPR1-2 | This study |
| NPR1M2B | NPR1M1B | NPR1-1/npr1-2::FRT | This study |
| NPR1M3A | NPR1M2A | npr1-1::FRT/npr1-2::SAT1-FLIP | This study |
| NPR1M3B | NPR1M2B | npr1-1::SAT1-FLIP/npr1-2::FRT | This study |
| NPR1M4A | NPR1M3A | npr1-1::FRT/npr1-2::FRT | This study |
| NPR1M4B | NPR1M3B | npr1-1::FRT/npr1-2::FRT | This study |
| NPR1MK1A | NPR1M4A | NPR1-SAT1-FLIP/npr1-2::FRT | This study |
| NPR1MK1B | NPR1M4B | npr1-1::FRT/NPR1-SAT1-FLIP | This study |
| NPR1MK2A | NPR1MK1A | NPR1-FRT/npr1-2::FRT | This study |
| NPR1MK2B | NPR1MK1B | npr1-1::FRT/NPR1-FRT | This study |
| mep2Δ mutants | |||
| SCMEP2M4A | SC5314 | mep2-1::FRT/mep2-2::FRT | 6 |
| SCMEP2M4B | SC5314 | mep2-1::FRT/mep2-2::FRT | 6 |
| mep1Δ mep2Δ double mutants | |||
| SCMEP12M1A | SCMEP2M4A | MEP1-1/mep1-2::SAT1-FLIP | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| SCMEP12M1B | SCMEP2M4B | mep1-1::SAT1-FLIP/MEP1-2 | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| SCMEP12M2A | SCMEP12M1A | MEP1-1/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| SCMEP12M2B | SCMEP12M1B | mep1-1::FRT/MEP1-2 | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| SCMEP12M3A | SCMEP12M2A | mep1-1::SAT1-FLIP/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| SCMEP12M3B | SCMEP12M2B | mep1-1::FRT/mep1-2::SAT1-FLIP | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| SCMEP12M4A | SCMEP12M3A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| SCMEP12M4B | SCMEP12M3B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| mep1Δ mep2Δ mep3Δ triple mutants | |||
| SCMEP123M1A | SCMEP12M4A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| mep3-1::SAT1-FLIP/MEP3-2 | |||
| SCMEP123M1B | SCMEP12M4B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| MEP3-1/mep3-2::SAT1-FLIP | |||
| SCMEP123M2A | SCMEP123M1A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| mep3-1::FRT/MEP3-2 | |||
| SCMEP123M2B | SCMEP123M1B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| MEP3-1/mep3-2::FRT | |||
| SCMEP123M3A | SCMEP123M2A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| mep3-1::FRT/mep3-2::SAT1-FLIP | |||
| SCMEP123M3B | SCMEP123M2B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| mep3-1::SAT1-FLIP/mep3-2::FRT | |||
| SCMEP123M4A | SCMEP123M3A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| mep3-1::FRT/mep3-2::FRT | |||
| SCMEP123M4B | SCMEP123M3B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| mep3-1::FRT/mep3-2::FRT | |||
| mep1Δ mep2Δ npr1Δ triple mutants | |||
| Δmep12NPR1M1A | SCMEP12M4A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| npr1-1::SAT1-FLIP/NPR1-2 | |||
| Δmep12NPR1M1B | SCMEP12M4B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| NPR1-1/npr1-2::SAT1-FLIP | |||
| Δmep12NPR1M2A | Δmep12NPR1M1A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| npr1-1::FRT/NPR1-2 | |||
| Δmep12NPR1M2B | Δmep12NPR1M1B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| NPR1-1/npr1-2::FRT | |||
| Δmep12NPR1M3A | Δmep12NPR1M2A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| npr1-1::FRT/npr1-2::SAT1-FLIP | |||
| Δmep12NPR1M3B | Δmep12NPR1M2B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| npr1-1::SAT1-FLIP/npr1-2::FRT | |||
| Δmep12NPR1M4A | Δmep12NPR1M3A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| npr1-1::FRT/npr1-2::FRT | |||
| Δmep12NPR1M4B | Δmep12NPR1M3B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| npr1-1::FRT/npr1-2::FRT | |||
| Strains expressing MEP1, MEP2, or MEP2G343C in mep1Δ mep2Δ or mep1Δ mep2Δ npr1Δ backgrounds | |||
| SCΔmep12MEP1K1A | SCMEP12M4A | mep1-1::FRT/MEP1-caSAT1 | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| SCΔmep12MEP1K1B | SCMEP12M4A | MEP1-caSAT1/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| SCΔmep12MEP2K1A | SCMEP12M4A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/MEP2-caSAT1 | |||
| SCΔmep12MEP2K1B | SCMEP12M4B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/MEP2-caSAT1 | |||
| SCΔmep12MEP2K2A | SCMEP12M4A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/MEP2G343C-caSAT1 | |||
| SCΔmep12MEP2K2B | SCMEP12M4B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/MEP2G343C-caSAT1 | |||
| Δmep1Δmep2Δnpr1MEP1K1A | Δmep12NPR1M4A | mep1-1::FRT/MEP1-caSAT1 | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| npr1-1::FRT/npr1-2::FRT | |||
| Δmep1Δmep2Δnpr1MEP1K1B | Δmep12NPR1M4B | MEP1-caSAT1/mep1-2::FRT | This study |
| mep2-1::FRT/mep2-2::FRT | |||
| npr1-1::FRT/npr1-2::FRT | |||
| Δmep1Δmep2Δnpr1MEP2K1A | Δmep12NPR1M4A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/MEP2-caSAT1 | |||
| npr1-1::FRT/npr1-2::FRT | |||
| Δmep1Δmep2Δnpr1MEP2K1B | Δmep12NPR1M4B | mep1-1::FRT/mep1-2::FRT | This study |
| MEP2-caSAT1/mep2-2::FRT | |||
| npr1-1::FRT/npr1-2::FRT | |||
| Δmep1Δmep2Δnpr1MEP2K2A | Δmep12NPR1M4A | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/MEP2G343C-caSAT1 | |||
| npr1-1::FRT/npr1-2::FRT | |||
| Δmep1Δmep2Δnpr1MEP2K2B | Δmep12NPR1M4B | mep1-1::FRT/mep1-2::FRT | This study |
| MEP2G343C-caSAT1/mep2-2::FRT | |||
| npr1-1::FRT/npr1-2::FRT | |||
| Strains expressing GFP-tagged MEP2 in mep1Δ mep2Δ or mep1Δ mep2Δ npr1Δ backgrounds | |||
| SCΔmep12MEP2G7A | SCMEP12M4A | mep1-1::FRT/mep1-2::FRT | This study |
| MEP2-GFP-caSAT1/mep2-2::FRT | |||
| SCΔmep12MEP2G7B | SCMEP12M4B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/MEP2-GFP-caSAT1 | |||
| SCΔmep12Δnpr1MEP2G7A | Δmep12NPR1M4A | mep1-1::FRT/mep1-2::FRT | This study |
| MEP2-GFP-caSAT1/mep2-2::FRT | |||
| npr1-1::FRT/npr1-2::FRT | |||
| SCΔmep12Δnpr1MEP2G7B | Δmep12NPR1M4B | mep1-1::FRT/mep1-2::FRT | This study |
| mep2-1::FRT/MEP2-GFP-caSAT1 | |||
| npr1-1::FRT/npr1-2::FRT |
a
SAT1-FLIP denotes the SAT1 flipper cassette. The NPR1, MEP1, MEP2, and MEP3 alleles in strain SC5314 can be distinguished by HaeII, ClaI, EcoRI, and BamHI restriction site polymorphisms, respectively. The NPR1 allele containing the polymorphic HaeII site at position +1002 was arbitrarily designated NPR1-2, the MEP1 allele with the polymorphic upstream ClaI site MEP1-1, the MEP2 allele with the polymorphic upstream EcoRI site MEP2-1, and the MEP3 allele with the polymorphic downstream BamHI site MEP3-2.