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Luciferase reporter assay. PflgK, a native promoter of csrABb, was fused to a modified luciferase gene within the plasmid pJSB161 (8). Luciferase activity was measured using a commercial luciferase assay kit and a Veritas microplate luminometer (Promega). The data are expressed as relative luciferase units per 106 cells (RLU/106 cells).
