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Heme and siderophore receptor expression levels by qPCR in receptor mutants. RNA was isolated from wild-type CFT073 and receptor mutants (indicated on x axis) cultured in LB containing 200 μM DPD. Transcript levels were measured in cDNA preparations from each strain and normalized to the gapA level, and results are shown as fold change relative to wild-type levels. Dashed lines represent 2-fold change and bars show the means.
