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. 2010 Dec 13;79(3):1098–1106. doi: 10.1128/IAI.01135-10

FIG. 8.

FIG. 8.

The kinetics of CD82 recruitment are similar to those of class II MHC recruitment to phagosomes containing C. neoformans. (A) Within 10 min after the addition of C. neoformans to DCs grown from class II MHC-eGFP mice and transduced to express CD82-mRFP1, phagosomes acquire both CD82-mRFP1 and class II MHC (left, class II MHC; center, CD82-mRFP1; right, merged image). Scale bar, 5 μm. (B and C) Time-lapse images in both red and green channels show that class II MHC-eGFP (B) appears around the phagosomes with kinetics similar to those of CD82-mRFP1 (C). Scale bar, 5 μm. (D) Kinetic analysis of fluorescence acquisition around phagosomes. Each data point (▵) represents the average intensity measured at each time point for at least 10 independent C. neoformans phagocytic events for CD82-mRFP1 over time (in minutes). The curves for class II MHC and CD63 are derived from identical experiments as described previously (2). a.u., arbitrary units. The error bars represent standard deviations.