TABLE 2.
Oligonucleotides used in this study
| Primer | Sequence (5′→3′)a | Coordinates in B31 chromosomeb | Purpose |
|---|---|---|---|
| BB0843F | TTATGGTATCAACTTTGGGATAACAG | 901760-901785 | Right-telomere ORF detection |
| BB0843R | ACAATAGAAAAGAAATAACCCAAAGC | 903285-903260 | |
| BB0843-4F | TCAGTTGTTATTGCCATGCAG | 903064-903084 | |
| BB0843-4R | TTAAAATCAAAGTCTTCTGTTAAAGG | 903902-903877 | |
| BB0844F | TTGCAATCTGTAATGCCTTTT | 903553-903573 | |
| BB0844R | GCAACAAGATGGTTTTGTTTCT | 905174-905153 | |
| BB0852F | AAAAGGATATGAAAATTATTCGCTAAA | 908198-908224 | |
| BB0852R | ATAGGGGCCTTTTACTTTTCTTGA | 909782-909759 | |
| BB0844outF | TTGTTTGATAGGATTAGAACAGAGG | 903496-903520 | Mutant construction or confirmation |
| BB0844outR | TGAATCGTTTTAAAAGGGGGTA | 905304-905283 | |
| BB0844EcoRIL | GGGAATTCCACATAGTAATTTAGCAATACAGGC | 904140-904116 | |
| BB0844EcoRIR | GGGAATTCCATCAACTAATTTAGCAAATTCAAGACTCCC | 904537-904567 | |
| flgBpF | TAATACCCGAGCTTCAAGGAAG | 304150-304129 | |
| aadAR | GACGTCATTATTTGCCGACTACC | NA | |
| 156a lp28-1F | GAGGCAGAGGATGCTTTAAACCAAT | NA | bb0844 localization in plasmid |
| 156a lp28-1R | AACTCTTAAGGCGTTGTAATTCGTAATTT | NA | |
| WI91-23 lp28-1R | CCTAATTTCCCGATGCTCTTCAAAAC | NA | |
| BB0844seqR | ACAAGCAGAGGTGCATGTTG | 904448-904429 | |
| FlaB-F | CTTTTCTCTGGTGAGGGAGCTC | 148259-148238 | Real-time RT-PCR |
| FlaB-R | GCTCCTTCCTGTTGAACACCC | 148288-148308 | |
| BB0844-574F | TTGCAGATGCTCTCTCGCTAAG | 904237-904258 | |
| BB0844-674R | GGCCGATTTTTACGTTTGTTATTT | 904158-904181 | |
| FL-Probe | FAM-CTTGAACCGGTGCAGCCTGAGCA-BHQ1 | 148264-148286 |
a
Underlining indicates an incorporated restriction site. FAM, 6-carboxyfluorescein.
b
NA, not applicable.