TABLE 1.
Strains examined in this study and summary of genotyping and virulence data
| Strain | Sourcea | rrn type | vcg genotype | rep-PCR | Capsule PCR | Lineage | MLST | Virulence group | No. with skin infection/totalb | Skin CFU count/g (log10)c | No. with liver infection/totalb | Liver CFU count/g (log10)c | No. dead/totalb | Temp (°C)c |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2400112 | C | B | C | 3 | 4 | 5/5 | 7.7 ± 1.2 | 5/5 | 5.0 ± 1.9 | 3/5 | 32.5 ± 1.7 | |||
| 106-2A | E | B | C | NGd | 2 | 3 | 4/5 | 6.9 ± 1.8 | 0/5 | 2.2 ± 0.1 | 0/5 | 37.8 ± 0.4 | ||
| 246-0058 Cape Canaveral | C | A | E | 7 | 0 | 3 | 5/5 | 6.8 ± 2.2 | 0/5 | 2.5 ± 0.0 | 0/5 | 36.1 ± 1.1 | ||
| 98-640 DP-E9 | E | A | E | 4 | 2 | 2 | 1 | 2 | 3/5 | 6.0 ± 2.8 | 0/5 | 2.5 ± 0.0 | 0/5 | 35.9 ± 0.7 |
| 98-641 DP-G8 | E | AB | E | 4 | 2 | 1 | 2 | 4/5 | 6.5 ± 2.5 | 0/5 | 2.5 ± 0.0 | 0/5 | 34.5 ± 0.6 | |
| 98-783 DP-A1 | E | A | E | 4 | 1 | 1 | 2 | 3/4 | 6.3 ± 2.2 | 0/4 | 2.5 ± 0.0 | 1/4 | 34.5 ± 1.3 | |
| 99-505 DP-C8 | E | AB | E | 4 | 2 | 1 | 2 | 5/5 | 6.2 ± 2.1 | 2/5 | 3.2 ± 0.9 | 1/5 | 34.6 ± 1.4 | |
| 99-509 DP-A6 | E | A | E | 4 | 1 | 2 | 4 | 8/10 | 6.7 ± 2.3 | 7/10 | 4.8 ± 2.4 | 4/10 | 33.5 ± 2.7 | |
| 99-520 DP-B8 | E | AB | E | 4 | 2 | 1 | 3 | 5/5 | 7.9 ± 0.6 | 0/5 | 2.5 ± 0.0 | 0/5 | 36.3 ± 0.4 | |
| 99-537 DP-G7 | E | A | E | 4 | 2 | 1 | 3 | 5/5 | 8.2 ± 0.3 | 2/5 | 3.0 ± 0.7 | 0/5 | 37.2 ± 1.2 | |
| 99-540 DP-B6 | E | A | E | 4 | 2 | 2 | 1 | 3 | 5/5 | 7.7 ± 0.4 | 0/5 | 2.5 ± 0.0 | 0/5 | 36.3 ± 0.6 |
| 99-578 DP-B1 | E | B | C | 3 | 1 | 2 | 4 | 5/5 | 7.9 ± 0.2 | 4/5 | 4.9 ± 1.4 | 4/5 | 32.3 ± 1.9 | |
| 99-581 DP-C7 | E | A | E | 6 | 2 | 1 | 3 | 5/5 | 7.2 ± 0.6 | 2/5 | 3.0 ± 0.6 | 0/5 | 36.8 ± 0.3 | |
| 99-584 DP-B12 | E | A | E | 4 | 2 | 1 | 2 | 4/10 | 4.6 ± 2.0 | 0/10 | 2.5 ± 0.0 | 0/10 | 35.2 ± 1.2 | |
| 99-609 DP-A4 | E | A | E | 4 | 2 | 2 | 1 | 3 | 5/5 | 7.9 ± 0.4 | 3/5 | 2.8 ± 0.3 | 1/5 | 35.1 ± 1.8 |
| 99-622 DP-E4 | E | A | E | 4 | 2 | 1 | 4 | 5/5 | 7.7 ± 0.3 | 2/5 | 3.6 ± 1.6 | 0/5 | 34.1 ± 1.1 | |
| 99-623 DP-F5 | E | AB | E | 4 | 2 | 1 | 2 | 5/5 | 5.4 ± 2.0 | 0/5 | 2.5 ± 0.1 | 0/5 | 35.5 ± 0.8 | |
| 99-624 DP-C10 | E | A | E | 4 | 2 | 1 | 3 | 4/4 | 6.8 ± 2.5 | 1/4 | 3.2 ± 1.3 | 1/4 | 35.7 ± 2.1 | |
| 99-625 DP-D8 | E | AB | E | 4 | 2 | 1 | 4 | 5/5 | 8.1 ± 0.2 | 3/5 | 4.0 ± 1.6 | 2/5 | 34.1 ± 3.3 | |
| 99-645 DP-C4 | E | A | E | 5 | 2 | 1 | 4 | 5/5 | 8.3 ± 0.4 | 4/5 | 4.7 ± 1.9 | 2/5 | 34.1 ± 2.0 | |
| 99-736 DP-C7 | E | AB | E | 4 | 2 | 1 | 2 | 7/10 | 6.1 ± 2.5 | 3/10 | 3.2 ± 1.1 | 2/10 | 34.3 ± 2.3 | |
| 99-738 DP-B5 | E | A | E | 4 | 2 | 1 | 5 | 5/5 | 8.3 ± 0.3 | 5/5 | 5.4 ± 1.0 | 2/5 | 33.5 ± 1.3 | |
| 99-742 DP-A9 | E | AB | E | 4 | 2 | 1 | 4 | 5/5 | 6.5 ± 2.0 | 3/5 | 4.4 ± 1.7 | 3/5 | 33.7 ± 1.7 | |
| 99-743 DP-B6 | E | B | E | 3 | 1 | 1 | 3 | 4/5 | 7.3 ± 2.5 | 0/5 | 2.5 ± 0.0 | 0/5 | 35.1 ± 1.6 | |
| 99-779 DP-D2 | E | A | E | 6 | 0 | 2 | 1 | 3 | 5/5 | 6.8 ± 2.5 | 1/5 | 3.4 ± 0.5 | 1/5 | 34.4 ± 2.4 |
| 99-780 DP-E1 | E | A | E | 5 | 1 | 1 | 3 | 5/5 | 7.2 ± 1.6 | 1/5 | 2.7 ± 0.5 | 0/5 | 35.4 ± 1.9 | |
| 99-796 DP-E7 | E | AB | E | 4 | 2 | 1 | 4 | 5/5 | 8.4 ± 0.3 | 5/5 | 4.5 ± 1.4 | 0/5 | 35.2 ± 2.2 | |
| ATCC 29307 | C | B | C | 4 | 5/5 | 8.1 ± 0.0 | 3/5 | 5.0 ± 2.6 | 0/5 | 34.6 ± 1.5 | ||||
| ATL-9572 | C | A | E | 3 | 2 | 1 | 1 | 0/5 | 3.0 ± 0.0 | 0/5 | 2.5 ± 0.0 | 0/5 | 35.4 ± 1.0 | |
| ATL-9580 | C | B | C | 3 | 1 | 2 | 5 | 5/5 | 8.3 ± 0.2 | 5/5 | 5.8 ± 0.0 | 5/5 | 29.6 ± 0.0 | |
| ATL-9824 | C | B | C | 4 | 1 | 2 | 2 | 3/5 | 6.1 ± 2.9 | 2/5 | 3.4 ± 1.2 | 1/5 | 34.7 ± 2.0 | |
| CDC 9003-97 (FLA 9509) | C | B | C | 9 | 1 | 2 | 4 | 5/5 | 8.3 ± 0.1 | 5/5 | 5.1 ± 0.5 | 5/5 | 29.7 ± 1.0 | |
| CDC 9005-97 (DAL 7-9087) | C | A | E | 3 | 2 | 1 | 1 | 3 | 9/9 | 7.0 ± 1.4 | 3/9 | 2.6 ± 0.3 | 0/10 | 36.0 ± 0.6 |
| CDC 9030-95 (ORL 1506) FLDHRS-383C | C | AB | E | 4 | 2 | 2 | 1 | 5 | 10/10 | 7.8 ± 0.3 | 10/10 | 5.7 ± 1.1 | 10/10 | 29.2 ± 1.4 |
| CDC 9031-96 (ATL 6-1306) | C | AB | E | 4 | 2 | 1 | 3 | 5/5 | 6.9 ± 1.9 | 3/10 | 2.7 ± 0.6 | 2/10 | 35.3 ± 2.6 | |
| CDC 9032-95 (ORL-8074) FLDHRS-407C | C | B | C | 3 | 1 | 2 | 4 | 5/5 | 7.9 ± 0.5 | 5/5 | 5.4 ± 0.1 | 5/5 | 29.9 ± 0.5 | |
| CDC 9038-96 (LOS 7318) | C | B | C | 2 | 1 | 1 | 2 | 2 | 5/5 | 6.1 ± 1.7 | 0/5 | 2.5 ± 0.0 | 0/5 | 34.9 ± 0.8 |
| CDC 9053-96 (FLA 8869) | C | B | C | 9 | 1 | 2 | 2 | 4/5 | 4.9 ± 2.1 | 1/5 | 2.8 ± 0.7 | 0/5 | 35.5 ± 0.6 | |
| CDC 9060-96 (DAL 7-9002) | C | B | C | 4 | 2 | 2 | 5 | 5/5 | 7.6 ± 0.5 | 5/5 | 6.3 ± 1.0 | 5/5 | 29.6 ± 1.2 | |
| CDC 9062-96 (LOS 7343) | C | B | C | 9 | 1 | 1 | 2 | 4 | 10/10 | 8.1 ± 0.9 | 6/10 | 3.5 ± 1.4 | 4/10 | 33.4 ± 3.6 |
| CDC 9067-96 (DAL 7-9000) | C | B | C | 9 | 1 | 2 | 4 | 5/5 | 7.9 ± 0.3 | 5/5 | 5.3 ± 1.1 | 4/5 | 31.8 ± 1.1 | |
| CDC 9070-96 (DAL 79040) | C | B | C | 4 | 1 | 2 | 2 | 3/5 | 5.9 ± 2.7 | 3/5 | 3.7 ± 1.2 | 2/5 | 34.0 ± 2.5 | |
| CDC 9074-96 (ATL 71491) | C | B | C | 9 | 1 | 2 | 5 | 5/5 | 8.2 ± 0.3 | 5/5 | 6.5 ± 1.4 | 5/5 | 27.9 ± 1.9 | |
| CDC 9075-96 (ATL 71503) | C | AB | E | 4 | 2 | 1 | 2 | 4/5 | 6.0 ± 2.5 | 2/5 | 3.7 ± 1.2 | 1/5 | 34.9 ± 3.3 | |
| CDC 9076-96 (ATL 71504) | C | B | C | 9 | 1 | 2 | 5 | 5/5 | 8.5 ± 0.1 | 5/5 | 5.4 ± 1.4 | 0/5 | 35.1 ± 2.1 | |
| CDC 9149-95 (NSV 5829) | C | AB | E | 4 | 2 | 1 | 4 | 5/5 | 8.0 ± 0.3 | 5/5 | 4.9 ± 1.5 | 3/5 | 33.0 ± 2.5 | |
| CDC 9340-95 (ORL 8324) FLDHRS-721C | C | B | C | 1 | 1 | 2 | 4 | 5/5 | 8.0 ± 0.3 | 4/5 | 4.2 ± 1.3 | 3/5 | 32.9 ± 3.2 | |
| CDC 9342-95 (LOS 6966) | C | B | C | 4 | 2 | 2 | 2 | 3/5 | 5.9 ± 2.6 | 0/5 | 2.5 ± 0.1 | 0/5 | 35.8 ± 1.1 | |
| CDC 9345-95 (DAL 6-5000) | C | B | C | NG | 2 | 2 | 1 | 5/5 | 4.0 ± 1.3 | 1/5 | 2.6 ± 0.1 | 0/10 | 36.0 ± 0.5 | |
| CDC 9348-95 (NSV 5830) | C | AB | E | 4 | 2 | 1 | 5 | 5/5 | 8.1 ± 0.0 | 5/5 | 6.7 ± 0.2 | 4/5 | 31.1 ± 1.1 | |
| CDC 9349-95 (NSV 5736) | C | B | C | 3 | 1 | 2 | 5 | 5/5 | 8.3 ± 0.5 | 5/5 | 7.3 ± 0.9 | 4/5 | 31.6 ± 0.0 | |
| CDC 9352-94 (ATL 9823) | C | B | C | 1 | 1 | 2 | 4 | 5/5 | 8.4 ± 0.5 | 3/5 | 3.3 ± 0.8 | 0/5 | 37.3 ± 1.0 | |
| CMCP6 | C | B | C | NG | 1 | 1 | 2 | 4 | 5/5 | 7.2 ± 0.3 | 5/5 | 4.3 ± 1.2 | 4/5 | 31.2 ± 2.0 |
| FDOH 6325 | C | A | E | 7 | 0 | 4 | 7/10 | 6.5 ± 2.4 | 7/10 | 4.2 ± 1.2 | 6/10 | 31.3 ± 4.3 | ||
| LL728 | C | B | C | NG | 4 | 4/5 | 7.5 ± 2.1 | 4/5 | 4.8 ± 1.3 | 4/5 | 33.2 ± 1.9 | |||
| MO6-24/O | C | B | C | 9 | 1 | 1 | 2 | 4 | 5/5 | 7.9 ± 0.1 | 5/5 | 5.2 ± 0.7 | 5/5 | 29.0 ± 2.1 |
| MLT365 | E | B | C | 8 | 1 | 1/5 | 3.7 ± 1.7 | 1/5 | 2.5 ± 0.1 | |||||
| MLT367 | E | B | C | 8 | 1 | 2/6 | 3.5 ± 1.1 | 0/6 | 2.5 ± 0.0 | |||||
| MLT403 | E | B | C | NG | 1 | 1/5 | 3.5 ± 1.1 | 0/5 | 2.5 ± 0.0 | |||||
| S1-10 | E | B | C | 9 | 1 | 2 | 2/5 | 5.1 ± 2.9 | 1/5 | 2.9 ± 1.1 | 0/5 | 37.5 ± 1.7 | ||
| S1-13 | E | A | E | 6 | 1 | 4 | 5/5 | 8.1 ± 0.2 | 4/5 | 4.7 ± 1.9 | 4/5 | 32.5 ± 3.8 | ||
| S1-8 | E | A | E | 4 | 0 | 2 | 0/5 | 4.5 ± 0.0 | 0/5 | 2.5 ± 0.0 | 0/5 | 37.4 ± 0.5 | ||
| S2-10 | E | A | E | 3 | 0 | 4 | 5/5 | 8.3 ± 0.4 | 2/5 | 3.4 ± 1.7 | 2/5 | 35.8 ± 3.9 | ||
| S2-20 | E | B | C | 3 | 1 | 4 | 4/4 | 8.2 ± 0.3 | 4/4 | 4.8 ± 0.7 | 4/4 | 32.6 ± 3.4 | ||
| S2-22 | E | B | C | 9 | 1 | 3 | 4/5 | 6.8 ± 1.9 | 1/5 | 2.5 ± 0.7 | 0/5 | 38.3 ± 0.3 | ||
| S3-16 | E | B | C | 3 | 0 | 1 | 1/5 | 3.2 ± 0.0 | 0/5 | 2.4 ± 0.2 | 0/5 | 36.8 ± 0.6 | ||
| S3-5 | E | A | E | 4 | 0 | 4 | 5/5 | 7.5 ± 1.3 | 4/5 | 4.7 ± 1.1 | 2/5 | 34.5 ± 2.1 | ||
| VV1009 | C | B | C | NG | 4 | 4/5 | 6.9 ± 2.3 | 4/5 | 4.6 ± 1.3 | 3/5 | 34.3 ± 3.1 | |||
| YJ016 | C | B | C | 2 | 2 | 1 | 2 | 5 | 5/5 | 7.7 ± 0.3 | 8/8 | 5.7 ± 0.2 | 8/8 | 29.1 ± 3.6 |
a
C, clinical; E, environmental.
b
Number of mice with detectable skin infection, liver infection, or a body temperature of less than 33°C over the total number of mice inoculated.
c
Mean ± standard deviation.
d
NG, not grouped. No other strains exhibited greater than 85% homology.