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Impacts of VirB8 interaction inhibitors on VirB protein levels in B. abortus 2308. Brucella was cultivated in a virulence gene-inducing minimal medium in the presence of small molecules, as indicated. C, DMSO control; HSL, C12-homoserine lactone; and VirB8 interaction inhibitors (B8I- plus numbers above lanes). B. abortus cells were lysed, followed by SDS-PAGE and Western blotting with specific antisera as indicated.
