TABLE 4.
Impacts of VirB8 interaction inhibitors on intracellular replication in macrophagesa
| Compound | Concn (μM) | Avg replication | SD |
|---|---|---|---|
| DMSO | NA | 4.98 | 0.20 |
| C12-HSL | 5 | 0.17 | 0.41 |
| B8I-1 | 12.5 | 5.18 | 0.31 |
| B8I-1 | 5 | 5.36 | 0.20 |
| B8I-2 | 12.5 | 1.17 | 1.61 |
| B8I-2 | 8 | 1.17 | 1.83 |
| B8I-2 | 5 | 3.85 | 0.23 |
| B8I-2 | 4 | 4.09 | 0.37 |
| B8I-2 | 1 | 4.84 | 0.26 |
| B8I-3 | 12.5 | 5.56 | 0.25 |
| B8I-3 | 5 | 5.44 | 0.31 |
| B8I-4 | 5 | 4.61 | 0.39 |
| B8I-6 | 12.5 | 4.46 | 0.40 |
| B8I-6 | 5 | 4.39 | 0.44 |
| B8I-8 | 12.5 | 3.99 | 0.60 |
| B8I-8 | 5 | 4.33 | 0.57 |
| B8I-9 | 12.5 | 4.74 | 0.53 |
| B8I-9 | 5 | 4.69 | 0.46 |
| B8I-10 | 12.5 | 4.87 | 0.44 |
| B8I-10 | 5 | 4.71 | 0.51 |
| B8I-11 | 12.5 | 3.99 | 0.67 |
| B8I-11 | 5 | 4.48 | 0.73 |
| B8I-12 | 12.5 | 4.23 | 0.85 |
| B8I-12 | 5 | 4.13 | 0.41 |
| B8I-14 | 12.5 | 4.73 | 0.60 |
| B8I-14 | 5 | 4.33 | 0.83 |
| B8I-15 | 12.5 | 5.15 | 0.33 |
| B8I-15 | 5 | 5.18 | 0.26 |
| B8I-16 | 12.5 | 5.13 | 0.05 |
| B8I-16 | 5 | 5.16 | 0.07 |
| B8I-17 | 12.5 | 5.13 | 0.20 |
| B8I-17 | 5 | 5.18 | 0.24 |
| B8I-20 | 12.5 | 5.53 | 0.27 |
a
Intracellular growth of B. abortus 2308 after 48 h was quantified by lysis of macrophages, dilution, and plating (replication is shown on a logarithmic scale). Averages from three replicates with three wells per replicate are shown. NA, not applicable.