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Southern hybridization analysis of tcsL mutants. Genomic DNA was purified and digested with HaeIII and EcoRI prior to electrophoresis on 0.8% agarose gels. The Southern blots were probed with probes specific for (A) tcsL, (B) ermB, and (C) catP. WT, wild-type ATCC 9714; DLL5001 to DLL5003, tcsL mutants; V, vector control pDLL2; M, DIG-labeled λ-HindIII molecular size markers (kb).
