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. 2010 Dec 13;79(3):1349–1362. doi: 10.1128/IAI.00984-10

FIG. 2.

FIG. 2.

il17ra−/− mice display reduced IFN-γ and IL-12p70 production in the iliac nodes. Iliac node mononuclear cells from individual il17ra−/− mice or C57BL/6 mice intravaginally infected with C. muridarum were stimulated in vitro with UV-inactivated EBs for 96 h. The supernatants were analyzed for IFN-γ (A), IL-12p70 (B), and IL-4 (C). Levels of IFN-γ and IL-12p70 were significantly reduced in il17ra−/− mice. Addition of a blocking anti-CD4 antibody resulted in >95% inhibition of the IFN-γ response, and the levels were undetectable in cells incubated with medium alone (data not shown). The data points represent the means and SD of values from 5 mice in a single independent experiment. *, P < 0.050; **, P < 0.001 (Student's t test).