Skip to main content
. 2010 Dec 20;79(3):1338–1348. doi: 10.1128/IAI.01188-10

FIG. 1.

FIG. 1.

Invasion of Borrelia burgdorferi into fibroblast cells. A total of 1 × 106 L929 fibroblasts were cultured and infected with B. burgdorferi strain ML23/pBSVφ at an MOI of 5 for 2 h. After the cells were washed and fixed, the samples were incubated with mouse anti-OspA antibody and an Alexa 594-tagged secondary antibody. Total GFP fluorescence (A), detection of only the Alexa 594 signal (B), and merge of images (C) shown in panels A and B. Arrows in panel C denote intracellular B. burgdorferi.