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Lack of OprF alters OM physicochemical properties. Gentamicin-assisted lysozyme lysis of strains H103 (wild type; black squares), H636 (oprF mutant; white squares), and H636O (OprF-complemented mutant; gray diamonds) is shown. Results are the means of three independent experiments using 10 μg gentamicin ml−1 and 50 μg lysozyme ml−1, and the standard deviations were lower than 15%. Strains were grown in LB medium, and lysis was carried out in 5 mM HEPES.
