FIG. 8.
(A) Comparison of primary attachment to hydrophilic polystyrene by untreated BH1CC cells grown overnight in BHI glucose medium with cells pretreated for 2 h with 1 mg/ml DNase I or heat-inactivated DNase I (control). BH1CC Δatl::Cmr was included as a control. Primary attachment is expressed as the percent attachment after the cultures were washed. Experiments were repeated three times, and standard deviations are indicated. (B) Biofilm formation by isolates 8325-4 and BH1CC grown for 24 h in BHI glucose medium in hydrophilic 96-well polystyrene plates in the absence and presence of 0.5 mg/ml DNase I. The results are representative of those from three independent experiments, and standard deviations are indicated. (C) Susceptibility of 24-h BH1CC biofilms to dispersal by DNase I (0.5 mg/ml).