FIG. 4.

Inhibitory effect of MyD88 and TRAF6 gene silencing in serovar Shermani detergent extract-induced increase in fibronectin production. HK-2 cells were transfected with MyD88 (A and B), TRAF6 (C and D), or scramble siRNA (control) at different concentrations (1 to 200 nM) for 24 h. Cells were then stimulated with serovar Shermani detergent extract (0.1 μg/ml) under serum-free condition for further 2 days. Fibronectin gene expression was assessed by real-time PCR (A and C), and protein production was determined by Western blot analysis (B and D). The results of real-time PCR are expressed as the mean relative fold increase in fibronectin gene expression over that of the untreated group ± the SE of triplicate measurements from four independent experiments (*, P < 0.05). The results of one representative result of Western blot analysis of three individual replicate experiments are shown.