FIG. 5.

Suppressive effect of NF-κB inhibition on the serovar Shermani detergent extract-induced increase in fibronectin production. (A) HK-2 cells were treated with serovar Shermani detergent extract (0.1 μg/ml) in the presence or absence of TLR2 or TLR4 siRNA for 90 min. Mock transfection with scramble siRNA was used as a negative control (cont.). Administration of Pam(3)CsK(4) (5 μg/ml) to cells was used as an internal positive control. The NF-κB transcription factor binding to its consensus oligonucleotide was quantified by EMSA as described in Materials and Methods. (B) Cells were stimulated with serovar Shermani detergent extract (0.1 μg/ml) in the presence or absence of different concentrations of curcumin (1 to 30 μM) (B) or QNZ (10 nM) (C) for 2 days. Fibronectin production was determined by Western blot analysis. The results of one representative experiment of at least three individual replicate experiments are shown.