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. 2011 Jan 3;79(3):1044–1056. doi: 10.1128/IAI.01118-10

FIG. 3.

FIG. 3.

Plasmid-cured C. trachomatis fails to elicit TLR2-dependent cytokine secretion. (A and B) C. trachomatis CTD153 induces weak or absent expression of TNF-α (A) or IL-6 (B) by DCs. Supernatants from murine BMDDCs cultured for 24 h at an MOI of 1 or 3 with D/UW-3/Cx or CTD153, or with the positive control TLR2 ligand, Pam3Cys, were assayed for TNF-α and IL-6 by bead array. Bars indicate means ± SD, calculated from triplicate wells in a single representative experiment (P < 0.001 at MOI of 3). (C and D) Vaginal secretions from mice infected with CTD153 also contained lower levels of TNF-α (P = 0.043 days 3 to 8) (C) or IL-6 (P = 0.013) (D) than those collected from mice infected with D/UW-3/Cx. This experiment was performed twice. Cytokines were assayed by bead array, and data points represent the means ± SD for each group of five mice.