FIG. 5.

PRCL transcription is reduced in plasmid-deficient C. trachomatis clinical isolates. C. trachomatis 25667R genes are identified according to the annotation for strain L2/434/Bu; thus, CTL0305 is homologous to CT049, CTL0339 to CT084, CTL0397 to CT142, CTL0638 to CT382.1, and CTL071 to CT702. C. trachomatis E/Bour and CT599 genes were assayed with the primers directed against strain D/UW-3/Cx and are identified according to that annotation, because neither serovar E strain has been sequenced. Total RNA was isolated from infected cells 24 h after infection, and PRCL transcripts were measured by quantitative RT-PCR. The transcriptional differences are presented as the fold change in expression for each gene from a single representative experiment. Each experiment was performed independently at least twice, and samples were assayed in triplicate. *, P ≤ 0.011 (L2/434/Bu versus 25667R) or P ≤ 0.006 or less (E/Bour versus CT599).