Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Expression of Pls1 is reduced in C. trachomatis CTD153 EBs. EBs of C. trachomatis D/UW-3/Cx and CTD153 were harvested from infected L929 cells 40 h after infection, gradient purified, and then lyzed by sonication. Immunoblotting was performed by using rabbit polyclonal anti-Pls1 antibody (1:10). As a loading control, Mip, the transcription of which is not altered in the absence of the chlamydial plasmid, was detected by using a rabbit anti-MIP antibody (1:100) (lower panel).
