FIG. 7.

Glycogen synthase expression is not sufficient for accumulation of glycogen by C. muridarum. Glycogen synthase expression is reduced in cytoplasmic extracts of plasmid-deficient C. trachomatis 25667R harvested 24 h after infection compared to extracts of L2/434/Bu, a representative plasmid-containing strain of the same serovar (A), but it is normally expressed in extracts of C. muridarum Nigg and CM3.1 (B), although neither plasmid-deficient strain accumulates glycogen within its inclusions (31). Enzyme assays were performed in a linear range (enzyme concentration versus rate, under steady-state conditions) during the time course of the assay. Data points represent the averages of at least two determinations that differed by less than 5% (error bars) in a single experiment.