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. 2011 Jan 3;79(3):1044–1056. doi: 10.1128/IAI.01118-10

FIG. 9.

FIG. 9.

C. trachomatis displays reduced TLR2 activation when cultured within 2DG-treated cells. (A) HEK-Blue-2 cells stimulated with D/UW-3/Cx and CTD153 or with D/UW-3Cx harvested from 2DG-treated L929 cells at an MOI of 0.5 or 3.0. After 24 h of incubation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue. *, P < 0.05 for D/UW-3/Cx versus CTD153 or D + 2DG at an MOI of 0.5 or 3.0; @, P < 0.05 for CTD153 versus D + 2DG at an MOI of 3.0 by one-way ANOVA. (B) HEK-Blue-2 cells stimulated with Nigg and CM3.1 or with Nigg harvested from 2DG-treated L929 cells at an MOI of 3.0. *, P < 0.05 for Nigg versus CM3.1 at an MOI of 3.0 by one-way ANOVA. The TLR2 agonist Pam3Cys was used as a positive control. The data presented are from a single representative experiment, although each experiment was performed independently at least twice and samples were assayed in triplicate.