FIG. 4.

Functional state of neutrophils on day 1 after AP induction. (A) The MFI of the CD11b activation marker was measured in peripheral and peritoneal neutrophils (Ly-6G cells) by flow cytometry. The horizontal line represents the MFI value for untreated healthy mice (n = 9 mice per group; *, P < 0.05 versus sham group). (B) The adhesive properties of neutrophils were evaluated by measuring in vivo the percentage of cells within lung vessels that were positive for the myeloid marker Ly-6G, as described in Materials and Methods (n = 6 mice per group; *, P < 0.05 versus sham group). Representative dot plots are shown. (C) Representative photographs (May-Grünwald-Giemsa staining) showing toxic alterations on peripheral neutrophils from AP animals.