TABLE 2.
PCR primers used in this study
| Primer name | Sequence (5′-3′) | Target |
|---|---|---|
| 1498-up | ATGCTGGCCGGAATATATCTC | BPSS1498 |
| 1498-dn | GCCATTCGTCCAGTTTGCGG | BPSS1498 |
| 0518-up | ATGGCAAATGCTTTGGTTGA | BPSS0518 |
| 0518-dn | GATCGGCGCGTTCTGCTTCA | BPSS0518 |
| 2098-up | ATGGGCGTCGCAATGTTTATG | BPSS2098 |
| 2098-dn | CATCTCGGTGTTCTCCTTGATG | BPSS2098 |
| 0171-up | ATGGCGCAGGATATTTTTCTG | BPSS0171 |
| 0171-dn | GGCTTCCTTGTTGCCCTTGATG | BPSS0171 |
| 0099-up | ATGTCGCACGACATTTTCCTC | BPSS0099 |
| 0099-dn | CGCCGCGTTCTTCTTGATGTC | BPSS0099 |
| 3105-up | ATGTTACACATGCATTTGAAG | BPSS3105 |
| 3105-dn | GACCGCGTAGGTCTTGTCGTTC | BPSS3105 |
| BPSS1498-1 | GCTAGCATCCGCCAGTACGTCGTCGAC | BPSS1497 |
| BPSS1498-2 | GGATCCTTTAAATCTAGACGGCGACGATCTGTCCATTTC | BPSS1498 |
| BPSS1498-3 | TCTAGATTTAAAGGATCCCCGCACAAAGACGATAGCAAC | BPSS1498 |
| BPSS1498-4 | GCTAGCTCAGGAAATCGTTCGGATATC | BPSS1499 |
| BPSS0518-1 | GCTAGCATCTTCGCGACCTGCCGTTTC | BPSS0517 |
| BPSS0518-2 | GGATCCTTTAAATCTAGACTGCCAGCTCTGGATCTGGAT | BPSS0518 |
| BPSS0518-3 | TCTAGATTTAAAGGATCCACGGATCAGATCCGCATCAAC | BPSS0518 |
| BPSS0518-4 | GCTAGCATAACCACTCAGATAGTCGAG | BPSS0519 |
| BPSS2098-1 | GCTAGCCTCGCGAAGAACGGCTTCATG | BPSS2099 |
| BPSS2098-2 | GGATCCTTTAAATCTAGAGAACGACTGGATGTCGGTCCA | BPSS2098 |
| BPSS2098-3 | TCTAGATTTAAAGGATCCCAGATCGAGTTCATGCGCGTG | BPSS2098 |
| BPSS2098-4 | GCTAGCCTCGAACATCAGCACGTTGTG | BPSS2097 |
| BPSS0171-1 | GCTAGCGCCGAATACGACGATTCCGAC | BPSS0172 |
| BPSS0171-2 | GGATCCTTTAAATCTAGAGTGCATCGTCGATTCCTGCTG | BPSS0171 |
| BPSS0171-3 | TCTAGATTTAAAGGATCCGTCACGACGAGCTTCGACATC | BPSS0171 |
| BPSS0171-4 | GCTAGCCATTTTCCGGGTTCGGTGATC | BPSS0170 |
| BPSS0099-1 | GCTAGCCATCGAAAGAACACCGACTAC | BPSS0098 |
| BPSS0099-2 | GGATCCTTTAAATCTAGAGACCTCGATTTCGCCCTTGTG | BPSS0099 |
| BPSS0099-3 | TCTAGATTTAAAGGATCCCCGCTCGAATACATCAAGCTC | BPSS0099 |
| BPSS0099-4 | GCTAGCTTTCAGCTCGTACAGCCGCAC | BPSS0100 |
| BPSL3105-1 | GCTAGCCACTGCAAGAATTCCGATTAC | BPSL3106 |
| BPSL3105-2 | GGATCCTTTAAATCTAGACGGCTGAACGATCGAGTGATC | BPSL3105 |
| BPSL3105-3 | TCTAGATTTAAAGGATCCGAGATCAAGCTGAAGTACGTG | BPSL3105 |
| BPSL3105-4 | GCTAGCGACTGCTCGTTCAGCTCGAAC | BPSL3104 |
| amrR-1 | GCTAGCTCGGACAGGTCGAAGCCTTC | amrR |
| amrB-1 | GCTAGCACGATTTTCAGGCGGTTCTG | amrB |
| VgrG1-5′ | GATATCCAGGAGTTCCCGATGCCTTC | BPSS1503 |
| VgrG1-3′ | GATATCTCAGCCGAGCTGGATCAGTTG | BPSS1503 |
| 1503-up2 | GGATCCTCGTCGCACCGCAGCGTCGTC | BPSS1503 |
| 1503-dn2 | GGATCCTCAGCCGAGCTGGATCAGTTGGCC | BPSS1503 |