TABLE 3.
Results of vaccination of BALB/c mice with recombinant Hcp proteins and challenge with 50 MLD of B. pseudomallei K96243
| Antigena | No. of survivors/ total vaccinated | Bacteria present in the spleens of surviving animalsb |
||||
|---|---|---|---|---|---|---|
| Animal 1 | Animal 2 | Animal 3 | Animal 4 | Animal 5 | ||
| Hcp1 | 3/6 | 103 | 0 | 0 | NAc | NA |
| Hcp2 | 5/6 | >105 | >105 | 102 | 102 | 102 |
| Hcp3 | 3/6 | 102 | 103 | 103 | NA | NA |
| Hcp4 | 2/6 | 103 | 103 | NA | NA | NA |
| Hcp5 | 0/5 | NA | NA | NA | NA | NA |
| Hcp6 | 3/6 | 0 | 0 | 0 | NA | NA |
| Naïve | 0/6 | NA | NA | NA | NA | NA |
| SAS only | 0/6 | NA | NA | NA | NA | NA |
a
Recombinant Hcp proteins were mixed with SAS and delivered parentally three times over the course of a month. One mouse in the Hcp5 group died during vaccination, and so in that group only five animals were challenged with K96243.
b
Spleens were removed from euthanized animals on day 42 and immersed in 1 ml of PBS and homogenized, and 100-μl aliquots were plated to estimate the total number of bacteria present.
c
NA, not applicable.