TABLE 1.
Sequences of primers for microbiota composition and host gene expression analysis
| Targeta | Primer | Sequence | Tac (°C) |
|---|---|---|---|
| Eubacteria 16S rRNA (T-RFLP and cloning) | Bact-27fb | AGAGTTTGATCMTGGCTCAG | 55 |
| 926r | CCGTCAATTCCTTTRAGTTT | ||
| Eubacteria 16S rRNA (total bacteria) (1) | UniF340 | ACTCCTACGGGAGGCAGCAGT | 63 |
| UniR514 | ATTACCGCGGCTGCTGGC | ||
| Bacteroidales 16S rRNA (16) | BactF285 | GGTTCTGAGAGGAAGGTCCC | 61 |
| UniR338 | GCTGCCTCCCGTAGGAGT | ||
| Bifidobacterium 16S rRNA (30) | Bif164F | GGGTGGTAATGCCGGATG | 62 |
| Bif662R | CCACCGTTACACCGGGAA | ||
| Clostridium coccoides 16S rRNA (18) | UniF338 | ACTCCTACGGGAGGCAGC | 60 |
| CcocR491 | GCTTCTTAGTCAGGTACCGTCAT | ||
| Lactobacillus 16S rRNA (42) | LabF362 | AGCAGTAGGGAATCTTCCA | 56 |
| LabR677 | CACCGCTACACATGGAG | ||
| Muc2 (21) | MUC2-F | GCTGACGAGTGGTTGGTGAATG | 60 |
| MUC2-R | GATGAGGTGGCAGACAGGAGAC | ||
| TFF3 (21) | TFF3-F | CCTGGTTGCTGGGTCCTCTG | 60 |
| TFF3-R | GCCACGGTTGTTACACTGCTC | ||
| GAPDH | GAPDH-F | ATTGTCAGCAATGCATCCTG | 60 |
| GAPDH-R | ATGGACTGTGGTCATGAGCC |
a
Numbers in parentheses are references.
b
6-FAM-labeled 5′ end for T-RFLP.
c
Ta, annealing temperature.