FIG. 6.
Intestinal expression of NK cell and T cell markers in neonatal C57BL/6 and Rag2−/− mice during acute C. parvum infection. Neonatal C57BL/6 and Rag2−/− animals were infected by oral gavage with C. parvum oocysts, and intestinal tissue samples from the small intestine were collected 5 days postinfection. The amounts of intestinal IL-2Rβ (A), Klrb1b (B), CD69 (C), and granzyme B (D) mRNA were quantified by real-time quantitative PCR using the ΔΔCT method, with β-2-microglobulin as the reference gene and samples collected from uninfected animals as calibrators. The examples shown represent at least 6 animals per group (*, P < 0.05; **, P ≤ 0.01; ***, P < 0.001).