TABLE 1.
Salmonella strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| UK1 | Wild type | 38 |
| SL1344 | Wild type | 54 |
| LT2 | Wild type | 45 |
| ATCC 14028s | Wild type | ATCC |
| CAS152 | CS401 ΔsspB (sipB); Smr | 50 |
| YKJ013 | UK1 ΔsipB | 29 |
| YKJ032 | SL1344 ΔiacP | This study |
| YKJ033 | LT2 ΔiacP | This study |
| YKJ034 | UK1 iacP::Cm; Cmr | This study |
| YKJ035 | UK1 ΔiacP | This study |
| YKJ036 | ATCC 14028s ΔiacP | This study |
| YKJ074 | UK1 iacP::HA; Cmr | This study |
| YKJ204 | SL1344 sopE::Cm; Cmr | This study |
| YKJ205 | SL1344 ΔiacP sopE::Cm; Cmr | This study |
| YKJ029 | UK1 sopB::Km; Kmr | This study |
| YKJ099 | UK1 ΔiacP sopB::Km; Kmr | This study |
| YKJ042 | UK1 sopB::3×FLAG; Kmr | This study |
| YKJ098 | UK1 ΔiacP sopB::3×FLAG; Kmr | This study |
| YKJ119 | UK1 sptP::HA; Cmr | This study |
| YKJ123 | UK1 ΔiacP sptP::HA; Cmr | This study |
| Plasmids | ||
| pMW118 | Low-copy-no. plasmid; Apr | Nippon Gene |
| pBAD24 | Expression plasmid containing the arabinose-inducible promoter PBAD; Apr | 18 |
| pYKJ029 | pMW118 Psip; Apr | This study |
| pYKJ032 | pMW118 Pint-iacP; Apr | This study |
| pYKJ033 | pMW118 Psip-iacP::HA (piacP); Apr | This study |
| pYKJ034 | pBAD24 PBAD-iacP::HA; Apr | This study |
| pYKJ035 | pMW118 Psip-iacP(S38A)::HA; Apr | This study |
| pYKJ036 | pMW118 Psip-iacP(E51A)::HA; Apr | This study |
| pYKJ290 | pMW118 Psip-sipC::FLAG; Apr | This study |
| pYKJ291 | pMW118 Psip-sipD::FLAG; Apr | This study |
| pYKJ038 | pMW118 Psip-sipA::FLAG; Apr | This study |
| pYKJ304 | pBAD24 PBAD-sopA::FLAG; Apr | This study |
| pYKJ305 | pMW118 PsopD::FLAG; Apr | This study |
| pYKJ065 | pMW118 PsopE::HA; Apr | This study |