FIG. 1.

Mice fed a doubly selenium- and vitamin E-deficient diet have increased C. rodentium colonization of the colon and spleen. Mice (n = 7 or 8) were infected orally with approximately 1.0 × 10¹⁰ CFU of C. rodentium and sacrificed at 7, 12, and 17 days postinfection. The C. rodentium colon (A) and spleen (B) burdens were determined by plating serial dilutions of the tissue homogenates. Only within-day comparisons were made, using a t test (panel A and day 7 in panel B) or Mann-Whitney rank sum test (days 12 and 17 in panel B). The dashed line indicates the limit of detection. Within each day the mean (panel A and day 7 in panel B) or the median (days 12 and 17 in panel B) is indicated by a dash.