FIG. 3.

C. rodentium-infected mice fed doubly selenium- and vitamin E-deficient diet have increased colon pathology. (A, C, and E) Mice fed control adequate diet; (B, D, and E) mice fed doubly selenium- and vitamin E-deficient diet. (A and B) Uninfected mice. (C to F) Citrobacter rodentium-infected mice killed on day 12 (C and D) or day 17 (E and F). An equivalent 1-cm section of colon from each mouse was removed, opened longitudinally, pinned out mucosal side up, and fixed in buffered formalin. Samples were then processed for Giemsa staining. All photomicrographs were taken under identical conditions, and the bars indicate 100 μm.