. 2011 Jan 18;79(4):1471–1478. doi: 10.1128/IAI.01017-10

TABLE 1.

Colonic gene expression is altered in C. rodentium-infected mice fed a doubly selenium- and vitamin E-deficient diet

Gene product	Gene expression^a in:
	Uninfected mice fed adequate diet		Uninfected mice fed deficient diet		Infected mice fed adequate diet		Infected mice fed deficient diet
	C_T	Fold change	C_T	Fold change	C_T	Fold change	C_T	Fold change
IL-12p40	12.7 ± 1.1	1.0	13.5 ± 0.9	0.5	11.4 ± 0.7	2.4*	12.5 ± 1.3	1.1*#
IL-17A	16.2 ± 1.2	1.0	15.6 ± 1.1	1.5	11.0 ± 0.8	34.8*	10.2 ± 1.0	61.7*#
IL-22	17.0 ± 2.0	1.0	19.0 ± 1.7	0.3†	12.8 ± 2.0	18.4*	11.9 ± 1.1	34.9*
TNF-α	9.3 ± 0.5	1.0	9.5 ± 0.8	0.8	7.7 ± 0.8	3.0*	6.7 ± 0.8	6.0*#
iNOS	10.6 ± 0.6	1.0	11.0 ± 1.1	0.8	6.2 ± 1.2	21.2*	5.1 ± 1.3	46.2*#
IFN-γ	13.9 ± 0.9	1.0	14.9 ± 0.6	0.5†	10.6 ± 1.0	10.1*	9.1 ± 0.8	27.7*#
IL-6	14.1 ± 1.3	1.0	14.1 ± 1.7	1.0	12.9 ± 1.2	2.3*	10.2 ± 2.2	15.1*#
CCL2	10.1 ± 0.6	1.0	10.5 ± 0.6	0.7	7.9 ± 0.9	4.7*	6.4 ± 1.0	13.5*#
CXCL10	7.6 ± 0.8	1.0	8.5 ± 0.8	0.5†	5.5 ± 1.1	4.1*	3.7 ± 0.9	14.7*#
GPX1	2.9 ± 0.5	1.0	5.0 ± 0.4	0.2†	2.8 ± 0.5	1.0	4.9 ± 0.7	0.2#
GPX2	1.1 ± 0.4	1.0	1.1 ± 0.4	1.0	0.4 ± 0.5	1.7*	−0.4 ± 0.6	2.9*#
HMOX1	8.8 ± 0.7	1.0	8.1 ± 0.8	1.5	7.9 ± 0.8	1.9*	5.8 ± 0.9	7.8*#

C_T (mean ± SD) is defined as the threshold cycle; the lower the number, the higher the expression. The fold change in colonic gene expression at 12 days postinfection is in comparison to expression in uninfected mice fed a diet adequate in selenium and vitamin E. *, the value is significantly different (P < 0.05) from that for the corresponding uninfected group. #, the value is significantly different (P < 0.05) from that for the infected group fed an adequate diet. †, the value is significantly different (P < 0.05) from that for the uninfected group fed an adequate diet. The results were obtained by merging data from three separate experiments (n = 7 to 21).