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. 2011 Jan 31;79(4):1660–1670. doi: 10.1128/IAI.00872-10

FIG. 4.

FIG. 4.

Quantitative transcript analysis from the etb gene of TY825 and its isogenic mutants compared to their growth curves. Cells were grown in TSB medium with shaking at 37°C. Cell growth was monitored by measuring turbidity at 660 nm (C and D). Total RNA was extracted from the cultures at the time points shown. The expression level was measured by LightCycler RT-PCR as described in Materials and Methods and quantified relative to that of the internal control gene gyrB (A and B). The data presented are mean values from four independent RNA isolations. Error bars denote standard deviations. (A and C) Wild-type strain TY825 and the saeR (FK204), arlR (FK206), and agrC (FK211) mutants; (B and D) wild-type strain TY825 and the sigB (FK216), sarA (FK218), and sarS (FK217) mutants.