TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequence (5′ to 3′)a |
|---|---|
| Standard sequencing | |
| TsoriF | TACGATGACGTCTTTTGCGCAGTCGGC |
| TsoriR | ATAGACGTCGTGAGAAACAGCGTACAG |
| TetLF | TTATTGCAATGTGGAATTCGGAACGG |
| TetLR | CCGGGAATTCCTGTTATAAAAAAAGG |
| CMF | TTCATATGCCGGCAATAGTTACCCTT |
| CMR | TTCATATGGATCTGGAGCTGTAATAT |
| MW0018F | AAAAGAAGGATACGATGTGTACTGTGC |
| MW0018R | GTAAACGACGAATCGTTACATCGACCG |
| MW0199F | GTAAAATTAAACCATGCTGACCATCGTCTC |
| MW0199R | ATAGCATTTTCTATGAGTGGCTGAAGC |
| MW0236F | GGCCCTTTTGTAGGTCTATTTGTTGGCG |
| MW0236R | CCAATCCTTCTGCAAGTTGACGTTCCAC |
| MW0621F | GACAATACTTTGTTTCAAGAATTGAAA |
| MW0621R | GGTAATAAGAATTTCTAATATAATCATTT |
| MW0668F | TTTGTCAAACCTATTTTGAATATGAAGG |
| MW0668R | GTATATGGACATTCACGGTATTAGCATC |
| MW1208F | TTTCTTTTATAGTGCTTTTGCCGTTCC |
| MW1208R | TATACTATCAATCTCTTCAATAAATGATGG |
| MW1305F | AGCAAGCTTTCTTGAATTGGAACTCAC |
| MW1305R | TTGTTTAAGCTTCACTATTATAACCCC |
| MW1446F | CCATGAAGCAAGTAATGGCCAAGAGGC |
| MW1446R | CACACGATTTAACTTTTCTCTAAGTCG |
| MW1637F | TGGATCCAGTAGATGACGAACATTCAA |
| MW1637R | TAAGCTTATGCTCTCGTAATGACTC |
| MW1790F | GCGTCGAATATCGTCAATTGTTCTCGTA |
| MW1790R | GTCCAACTGGCTTAAGTTGCC |
| MW1825F | GTGAAACGTTAGATTTATACCATACACTCG |
| MW1825R | TTTGTACCGTTTGAATGACGC |
| MW1962F | CACCCTTAAAGAGATGAAATACAAACG |
| MW1962R | TTCTTGGAACAATTCATGAATGCGTGG |
| MW2002F | GAACAAATCACCCTATCGTCCTAAAGGC |
| MW2002R | CCCAGCTTAATGCTTGTTCATGCTGTTG |
| MW2282F | ATTGATGCATACACACAACCAAGTGG |
| MW2282R | ATTTTTTTAATCTTTGGCGTAGTCGC |
| MW2314F | ACATATCAGTTGATTGATCAAGACAGGGG |
| MW2314R | GTACTACTCGATAAACAACCG |
| MW2545F | AAAATGGAATTACGGTGTTATTGTCG |
| MW2545R | AGGCGCGTCATGTTAACAGCTAATGTG |
| sigBF | CAAGAAGCTTAGAATACAGATGCACAGG |
| sigBR | GTTAAAGCTTAATGGTCATCTTGTTGCC |
| sigBFS | TATGACTAGTTATATAAAAAAAGAGCAG |
| sigBRB2 | AATAAAGATCTTCTATTGATGTGCTGC |
| sarAF | ATTTGGGTAGTAAGCTTTGACACAAC |
| sarAR | GGATTTGAAGCTTTGCAACATCAACTAGC |
| sarAFS | ATCGAACTAGTTGCATCAAATAGGGAG |
| sarARB2 | TCATAGATCTCCAAATTGCGCTAAAC |
| sarSF | TATAATCATTGAAGCATATATGTTTCG |
| sarSR | TTATTGAGAGCTCTAACAGTTTGAGGG |
| sarSFS | ATTAAAAACTAGTGCATATACAAGGAG |
| sarSRB2 | CACTTTAGATCTCAGCACACTTGCGT |
| Quantitative RT-PCR | |
| gyrBF | AGGTCTTGGAGAAATGAATG |
| gyrBR | CAAATGTTTGGTCCGCTT |
| etaF | TACAGTTCCGGGAAATTCT |
| etaR | CCCAATACCAACACCATAA |
| etbF | GTGGTAAAGGCGGACAACAT |
| etbR | TCAAATCGTTCCCCAAAGTG |
| saeRF | CTGTAAATGGTCACGAAGT |
| saeRR | GACATTCACGGTATTAGCA |
| agrAF | GCAGTGAAATTCGTAAGCAT |
| agrAR | CGAGTTCTTAATTCTGCTGGA |
| sarAF | CGTAATGAGCATGATGAAAG |
| sarAR | ATTTCGTTGTTTGCTTCAG |
| sarSF | CCACCATAAATACCCTCAAACT |
| sarSR | GTCTTGCTGCGCGTCAT |
a
Sequence letters in boldface represent restriction sites.