TABLE 3.
Time (h) | No. of mice (n = 5/group) with result after injection witha: |
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---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TY825 (wild type) |
etb mutant |
saeR mutant |
arlR mutant |
agrC mutant |
sigB mutant |
sarA mutant |
sarS mutant |
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Activity | Death | Activity | Death | Activity | Death | Activity | Death | Activity | Death | Activity | Death | Activity | Death | Activity | Death | |
0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 |
6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 0 | 0 | 2 |
7 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 5 | 0 | 1 | 5 |
8 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 3 | 5 | 0 | 1 | 5 |
Aliquots of bacterial cell suspension (108 bacteria ml−1) were administered subcutaneously to groups of 2-day-old ICR neonatal mice, and the occurrence of Nikolsky's sign (exfoliation activity) was monitored.