FIG. 2.

Increase in Ch25h expression promotes survival of L. monocytogenes-infected macrophages. Macrophages that express higher levels of Ch25h (A) have improved survival after 12 h of L. monocytogenes infection (MOI, 5) as measured by the CellTiter-Fluor assay. Reduction in Ch25h expression leads to an increase in infection-induced death (B). The extent of infection-induced DNA fragmentation was evaluated by TUNEL staining (C). Cells that overexpress Ch25h (HiC25-1) have fewer TUNEL-positive cells than the control cell lines or cell lines that express Ch25h shRNA (shC25-B). The ratio of TUNEL-positive to DAPI-stained cells (D and E) was determined by counting 200 DAPI-positive cells in each group. Objective magnification is 100×. (n = 3 ± SD, representative of results of three independent experiments. *, P < 0.05; **, P < 0.01.)