FIG. 7.

The effect of Salmonella infection on steroid hormone metabolism. (A) Schematic of the C₂₁ steroid hormone metabolic pathway. Colors indicate the organs in which a given hormone was affected by infection (5 days). Color coding is the same as that described for Fig. 2. The outer and inner membranes mentioned are mitochondrial. Masses (Da) for metabolites affected are shown in parentheses. Solid arrows indicate direct steps, and dashed arrows indicate multiple steps that are not shown. Enzymes involved in some of the reactions displayed are shown in italics. (B) Levels of masses affected by infection (5 days) in the steroid pathway. Masses are shown at the top of each graph. The y axis indicates mass signal intensity. Signal/noise ratios for the metabolites shown varied between 7.4 and 125.9. Color coding is the same as that described for Fig. 2. Black bars represent uninfected samples, and gray bars indicate infected samples. Averages with standard errors of the means are shown. Four samples were used for feces and three for livers, with duplicate measurements (n = 8 for feces and n = 6 for livers). Ions presumptively neutralized to masses of 362.2093 and 364.225 Da using our method were detected in livers in both negative- and positive-ionization modes, and all results were used (n = 12). All P values were ≤0.0001, except for the metabolite with a mass of 364.225 Da in feces (P = 0.0065).