FIG. 4.

C. sakazakii Cpa is required for survival in NHS. (A) C. sakazakii and recombinant DH5α strains were incubated with 12 and 25% of NHS. (B) C. sakazakii BAA-894 and BAA894Δcpa strains were incubated with 25% CCPIS and 25% ACPIS. A serum survival assay was performed as described in Materials and Methods. The percent survivals shown are the means ± the standard deviations of three different assays. The P value was <0.05 for comparison of BAA-894 and BAA894Δcpa survival in 25 and 12% NHS as calculated by using the Student t test.