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Effect of lptC mutations on LptC-LptA interaction. Whole-cell extracts from AM604 cells transformed with plasmids pGS108 carrying the wild-type protein (LptC-H) or the LptC-mutated proteins LptCG56V-H, LptCG153R-H, and LptCΔ177-191-H were subjected to affinity chromatography. Equal amounts (2.5 μg) of Ni-NTA column-enriched LptC-H and its mutant versions were separated by SDS-12.5% PAGE and analyzed by Western blotting with anti-LptA antibody and HisProbe-HRP, respectively.
