FIG. 9.

LptA level upon LptD and LptE depletion. (A and B) Growth curves of AM661 (araBp-lptD [A]) and AM689 (araBp-lptE [B]). Cells growing exponentially in LD containing arabinose were harvested, washed, and subcultured in arabinose-supplemented (⧫) or arabinose-free (⋄) medium. Growth was monitored by measuring the OD₆₀₀. (C) Steady-state levels of LptA. Samples for protein analysis taken from cells grown in the presence (+) or absence (−) of arabinose at 210 min after the shift into nonpermissive condition were analyzed by Western blotting with anti-LptA and anti-LptE antibodies. Equal amounts of cells (0.2 OD₆₀₀ units) were loaded into each lane. AcrB was used as the loading control.