TABLE 2.
Oligonucleotides
| Name | Sequence (5′-3′)a | Use and/or description |
|---|---|---|
| AP24 | cgactagtctagaTTAAGGCTGAGTTTGTTTG | Random mutagenesis with AP54; XbaI |
| AP54 | cgagaggaattcaccATGAGTAAAGCCAGACGTTGGG | pGS108Δ177-191 construction, with AP172 and random mutagenesis with AP24; EcoRI |
| AP149 | atatacatATGAGTAAAGCCAGACGTTG | pET30-lptC-H construction, with AP150; NdeI |
| AP150 | cgcgcaggtaccAGGCTGAGTTTGTTTGTTTTG | pET30-lptC-H construction, with AP149; KpnI |
| AP164 | GTCTATAACCCAGAAGTGGCACTAAGCTATCG | pGS108G56V construction, with AP165 |
| AP165 | CGATAGCTTAGTGCCACTTCTGGGTTATAGAC | pGS108G56V construction, with AP164 |
| AP166 | cgagatggatccATGGCCGAAAAAGACGATAC | pQEsH-lptC construction, with AP167; BamHI |
| AP167 | cgagatctgcagTTAAGGCTGAGTTTGTTTG | pQEsH-lptC construction, with AP166; PstI |
| AP168 | CTCGTCACGTTATACAGAACAACATTTAACTC | pGS108G153R and pGS103G153R construction, with AP169 |
| AP169 | GAGTTAAATGTTGTTCTGTATAACGTGACGAG | pGS108G153R and pGS103G153R construction, with AP168 |
| AP172 | gtgatcacatctagatcagtggtggtggtggtggtgTTCAATCAGCTCGGCGTTC | pGS108Δ177-191 construction, with AP54; insertion of C-terminal His6 tag into LptCΔ177-191; XbaI |
a
Uppercase letters, E. coli genomic sequence; underlined lowercase letters, restriction sites; boldface letters, codons mutated by site-directed mutagenesis.